The study focuses on utilizing innovative liquid phase plasma technology for efficient hydrogen production from acetic acid, a key component of bio-oil. With a plate-pinhole-plate configuration, the researchers successfully initiated plasma to decompose acetic acid, overcoming challenges of discharge in high conductivity liquids. By analyzing the initiation process and electrical characteristics of the plasma, they optimized hydrogen production by investigating the effects of acetic acid concentration and discharge power. The results showed that at 60% acetic acid concentration and 302.8 W discharge power, the optimal hydrogen energy yield reached 1.7 mmol/kJ, with a maximum hydrogen flow rate of 678.7 mL/min. This performance surpasses many existing methods for hydrogen production from acetic acid. The study also delved into the gas and liquid phase products, as well as the reaction path of acetic acid decomposition. With the increasing focus on renewable clean energy sources to combat global climate change, hydrogen production from biomass, particularly acetic acid, holds significant promise. The use of plasma technology offers a catalyst-free, rapid-start solution that can be powered by renewable electricity, aligning with the push towards carbon neutrality and sustainable energy systems.