The study focuses on the potential of Staphylococcus epidermidis SPS3 in producing hydrogen (H2) from biowaste-based hydrolysates under dark fermentation. The bacterium achieved high H2 yields, showing promise for sustainable and efficient industrial H2 production. Through green-solvent pretreatment and enzymatic hydrolysis, substantial amounts of sugars were extracted from sawdust, contributing to enhanced H2 production. The research highlighted the advantages of using biomass-derived sugars over pure sugars for H2 production and addressed challenges such as lignin inhibition and enzyme effectiveness. The findings suggest that H2 production from biowaste via innovative approaches like the one demonstrated could be a game-changer in the renewable energy sector. Efforts are ongoing to optimize biomass valorization, pretreatment methods, and overall efficiency in dark fermentative H2 production processes. The study indicates a significant step towards cleaner energy generation and resource utilization from biowaste.